51�Թ�

The primer tries to connect itself to cDNA from the virus and make a copy.

The company’s test created cDNA, which is a clone or copy of the DNA, to test for the mutations.

Finally, to assess the effect of each variant on expression, we tested for a significant difference between the cDNA and gDNA alternate allele proportions with a two-sided t-test.

Approximately half of the cDNA product was used for library construction, and indexed libraries were generated using 18 cycles of PCR.

The protocol employed cDNA synthesis with random primers followed by gene specific multiplex PCR and is presented in detail in ref.